Not known Details About attune flow cytometer
Not known Details About attune flow cytometer
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Details-enriched characterization of mobile populations. Morphological info from illustrations or photos can increase to your richness of flow cytometry details. By way of example, the figure demonstrates an or else standard apoptosis assay working with Annexin V and PI, introducing mobile imaging to characterize cells in each inhabitants to reveal morphologically distinct functions. These insights couldn't are already acquired from multiplex staining on your own.
Regardless of whether you’re a novice or a specialist, examine our sources to progress your research and abilities in flow cytometry.
Merge acoustic concentrating technological innovation with superior-parameter analysis for versatile spectral unmixing and traditional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a significant-pace brightfield camera for simultaneous higher throughput flow cytometry and high resolution brightfield imaging.
*Pricing is indicative only and is particularly subject to vary. Remember to Get hold of your Agilent consultant with any questions. The knowledge established forth herein is not really an offer or perhaps a commitment, both of those of which are subject to credit approval, conditions and terms, and availability with Agilent. Agilent Financial Options is available in picked nations.
Right here, an experienced user has gated singlets confidently. Following analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of greater than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for improved workflow performance, and incorporate robotic integration for maximum throughput.
Steady impression good quality even at higher throughput Acoustic concentrating in addition to a significant-velocity digicam Blend to graphic these T cells constantly at small or higher flow charges. Simply modify concentrate and camera options to meet experimental requirements.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can rely on for consistent and trusted overall performance. The Attune CytPix product incorporates a higher-pace brightfield camera and AI-driven impression Evaluation program, with latest upgrades together with equipment Finding out applications and an intuitive person interface, enabling Increased data precision, accuracy, and more rapidly insights into sample biology.
People can look at celebration visuals in true-time to substantiate sample quality, improve protocols, and document the morphology of each mobile population recognized for Evaluation. Imaging capture also Gains from acoustic concentrating, which really helps to situation the cells to ensure a pointy, centered impression is attained.
AI-pushed automated graphic analysis Using the Attune Cytometric Program translates celebration options into distinct morphology parameters which might be combined with common fluorescence and scatter parameters to expose really reproducible population modifications and unique subsets.
Software note: Automated impression Evaluation lessens user-to-user variability in flow cytometry gating approaches
Constructed-in dimensionality reduction resources enhanced resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to confirm anticipated populace resolution in lysed full human blood. Person can check out events from Just about every cluster for Visible confirmation of mobile phenotypes.
Versatile payment plan from Agilent Economical Remedies permit you to get the important instrumentation you'll need for speedy undertaking, or to increase your lab.
Gating on these prolonged parameters lets you quickly and correctly identify populations of interest and confirm gating approach with little if any manual critique. The Attune program features usage of condition-of-the-art machine Studying Assessment tools in an expertly made consumer interface, making it extremely convenient to use.
As the mobile line had Earlier been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the photographs and have been able to doc the microbial infection at least five times earlier.
The Attune CytPix Flow Cytometer will allow users to visualise samples in the course of acquisition, confirm the accuracy of gating tactics, and assist effects with reliable documentation for publication reasons.
What gave the impression to be a homogeneous population is actually a lot more diverse—an insight that needs to be website thought of when interpreting effects.